Intein‐mediated creation of a cyclic acyl carrier protein
Bibliographic record
Abstract
Acyl carrier protein (ACP) is an essential protein in bacterial metabolism, where it acts as the major donor for fatty acyl groups in the synthesis of lipids and other specialized products. As such, ACP is an attractive anti‐microbial drug target. ACP normally encloses attached fatty acyl groups in a hydrophobic pocket within its three mainα‐helices, but the exact mechanism of interaction and acyl transfer from ACP to partner enzymes is poorly understood. ACP interacts with most proteins through its acidic helix II, and acyl transfer must involve partial or full unfolding of ACP in order to present the acyl chain to the partner enzyme. To examine the requirement of ACP unfolding for acyl transfer, we created a cyclic version of Vibrio harveyi ACP using split‐intein technology. Mass spectrometry, circular dichroism and fluorescence studies suggest that cyclic ACP (cACP) is trapped in a folded conformation. In vitro characterization indicates that cACP can be modified by holo‐ACP synthetase yielding an active ACP. Furthermore, in vivo complementation assays suggest that cACP is able to replace the linear wild‐type ACP and support growth. Our results thus suggest that cACP is able to interact with and transfer its acyl chain to its many partner enzymes within the bacterial cell despite constraining of its N‐ and C‐termini. (Supported by CIHR, NSERC, and an IWK Studentship).
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".