Proteomic Responses of Wheat Roots during Enhanced Ultraviolet-B Radiation Stress
Bibliographic record
Abstract
Enhanced ultraviolet-B (UV-B) radiation is one of the most important abiotic stresses that could influence the growth and physiological traits of plants. Wheat (Triticum aestivum) is also affected by present-day enhanced UV-B radiation. As protein metabolism plays an important role in plant adaptation to UV-B radiation, this study was designed to identify UV-B -responsive proteins in wheat roots. Wheat (Lin Fen NO.3) was exposed to UV-B radiation with normal light (group B) and only normal light (group CK) in growth chambers. Roots were harvested at 3d, 6d and 9d after UV-B radiation. Proteins were extracted and separated by two-dimensional gel electrophoresis (2-DE). 15 protein spots were regulated by UV-B radiation at 6d. Among these protein spots, 3 proteins were up-regulated and 10 proteins were down-regulated. 2 proteins were expressed only in CK group. 3 protein spots were identified using mass spectrometry. They were further verified as Ribulose-bisphosphate carboxylase activase; Catalase (CAT); Hypothetical protein. These data support the assumption that UV-B may have a regulatory role besides damaging effects and that an increased UV-B environment will likely increase this regulatory influence of UV-B radiation.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".