The role of reactive oxygen species and antioxidants during precooling stages of axis cryopreservation in recalcitrant <i>Trichilia dregeana</i>
Bibliographic record
Abstract
Cryopreservation is currently the only feasible method for long-term conservation of non-orthodox germplasm. Previous attempts to cryopreserve embryonic axes of Trichilia dregeana Sond. indicated that the inability to produce shoots, suggestedly associated with a wound-induced burst of reactive oxygen species (ROS) accompanied by declining antioxidant potential, consistently accompanied procedures necessary for cryopreservation. This study involved the provision of cathodic protection, both in the aqueous phase (electrolysis of a solution containing dilute electrolytes) and dry state (rapid drying of axes on a grid on which a static field is generated via the cathode of a power-pack) with the addition of other antioxidants, i.e., ascorbic acid and DMSO, to counteract uncontrolled ROS activity. The production of superoxide ([Formula: see text]), hydrogen peroxide (H 2 O 2 ), and hydroxyl radical ([Formula: see text]) in conjunction with the corresponding total aqueous antioxidant activity and viability was quantitatively assessed after each stage of the cryoprocedure, with or without cathodic protection and (or) antioxidants. No shoot production occurred in the treatments without cathodic protection and (or) antioxidants. In contrast, 80% of axes produced seedlings after excision, 40% after cryoprotection, and 40% after flash (rapid) drying, when the treatments included a form of cathodic protection.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".