Zeatin overcomes thidiazuron-induced inhibition of shoot elongation and promotes rooting in strawberry culture <i>in vitro</i>
Bibliographic record
Abstract
SummaryThe effects of 0, 2, 4 or 8 µM thidiazuron (TDZ) and explant type on adventitious shoots regenerated on excised leaf disks, sepals (calyx) and petiole halves of greenhouse-grown ‘Bounty’ strawberry (Fragaria ananassa Duch.) were studied. A moderate concentration (2 – 4 µM TDZ) supported bud and shoot regeneration without an intermediary callus phase on young expanding sepals, or on leaf disks with the adaxial side touching the culture medium, or on petiole halves placed horizontally with their cut-sides in contact with the medium, all maintained for 14 d in the dark. Sepals proved more effective than leaf discs and petiole segments for regenerating shoots. Another experiment compared the effects of 0, 0.5, 1, 2 and 4 µM TDZ or zeatin on shoot proliferation and rooting of sepal-derived adventitious shoots. Media containing TDZ generally promoted more callus formation and suppressed elongation and rooting of shoots. Shoots proliferated and roots developed best when explants were cultured in medium supplemented with 1 or 2 µM zeatin. An in vitro cloning protocol that enables shoot proliferation and rooting of strawberry explants in the same medium is expected to be applied for commercial micropropagation.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".