Monitoring calcium‐deficient calmodulin in mouse embryonic stem cells before and after cellular differentiation
Bibliographic record
Abstract
Calmodulin (CaM) is a calcium‐sensing protein that binds and regulates over 300 cellular proteins. Cellular localization and trafficking of calcium‐free CaM prior and after Ca 2+ stimulation is not well understood. It has been reported that 95% of total endogenous CaM is bound to target proteins in the cytoplasm, and that the influx of Ca 2+ results in its translocation to the nucleus. The present study was designed to investigate the Ca 2+ ‐ dependent and Ca 2+ ‐independent protein trafficking of CaM during cellular differentiation of mouse embryonic stem cell (MESC). Several CaM mutants were generated and labeled with a fluorescent marker for our investigation. In addition, a FITC labeled cell penetrating peptide (CPP) was used as a tool to introduce these CaM mutants into the cytoplasm of MESCs while maintaining high cell viability. The effects of these modifications on protein trafficking in MESCs during cellular differentiation are monitored via live fluorescent imaging and confocal microscopy. This research is supported by NSERC (J.G.G)
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".