Uptake, handling, and excretion of Na+ and Cl- from the diet <i>in vivo</i> in freshwater and seawater-acclimated killifish, <i>Fundulus heteroclitus</i>, an agastric teleost
Bibliographic record
Abstract
A radiotracer approach using diets labelled with (22)Na(+), (36)Cl(-) and [(14)C]polyethylene-4000 (PEG-4000) was employed to investigate the role of intestinal uptake from the food in ion homeostasis in the killifish Fundulus heteroclitus. This euryhaline teleost lacks both a stomach and the capacity for Cl(-) uptake at the gills in freshwater. PEG-4000 appearance in the water was minimal up to 10-11 h post-feeding, indicating the virtual absence of Na(+) and Cl(-) loss in the faeces up until this time. Rapid uptake of dietary Na(+) and Cl(-) occurred and more than 88% of (22)Na(+) and (36)Cl(-) were absorbed in the intestine by 3 h post-feeding; excretion rates of Na(+) and Cl(-) originating from the food were greatest during this period. Uptake and excretion of Cl(-) from the diet was fivefold to sixfold greater than that of Na(+) in freshwater, and approximately threefold greater in seawater. Excretions of dietary Na(+) and Cl(-) by seawater-acclimated killifish were far greater than by freshwater-acclimated killifish in this time frame, reflecting the much greater branchial efflux rates and turnover rates of the internal exchangeable pools. At both 3 and 9 h post-feeding, the largest fraction of dietary Na(+) was found in the carcass of freshwater-acclimated fish, followed by the external water, and finally the digestive tract. However, in seawater-acclimated fish, more was excreted to the water, and less was retained in the carcass. For Cl(-), which was taken up and excreted more rapidly than Na(+), the majority of the dietary load had moved to the external water by 9 h in both freshwater and seawater animals. After 7 days training on a low-salt natural diet (live Lumbriculus variegatus worms; 31.5 μmol Na(+) g(-1) wet mass) versus a high-salt synthetic pellet diet (911 μmol Na(+) g(-1) dry food mass), freshwater killifish exhibited a lower absolute excretion rate of Na(+) from the low-salt diet, but relative uptake from the intestine and retention in the carcass were virtually identical from the two diets. Seawater killifish excreted relatively more Na(+) from the low-salt diet. Overall, our results emphasize the importance of dietary Na(+) and Cl(-) in the electrolyte economy of the killifish, particularly in freshwater, and especially for Cl(-).
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".