Some fungi express β-1,3-glucanases similar to thaumatin-like proteins
Bibliographic record
Abstract
Mycelial clarified homogenates from Lentinus edodes, Agaricus bisporus, Rhizoctonia solani and extracts from commercial sources of lysing enzymes from Rhizoctonia solani or Irpex lacteus contained enzymes of ca 25 kDa hydrolyzing polymeric carboxy-methylated (CM)-pachyman with an in-gel β-1,3-glucanase assay. Proteins were purified from Rhizoctonia solani, Lentinus edodes and Irpex lacteus and their Nterminal amino acid sequences showed homology to thaumatin-like (TL) proteins. As with plant TL proteins with β-1,3-glucanase activity, little or no activity was observed on oligomeric laminarin by the in-gel hydrolase assay. The fungal TL proteins varied in specific activity from 60 to 760 nkat mg-1 with CM-pachyman as substrate and were thus more active than most plant TL proteins. Thin-layer chromatography indicated that the fungal TL proteins generated various oligoglucosides from an endo β-1,3-glucanase activity. The TL proteins were detected in several, but not all, anastomosis groups of Rhizoctonia solani. The purified Rhizoctonia solani TL protein was the most potent enzyme, inhibiting the growth of Saccharomyces cerevisiae when compared to two fungal or three plant TL proteins. Moreover, the Rhizoctonia TL protein was able to hydrolyze live Saccharomyces yeast cells. This is the first report of fungal TL proteins with endo-β-1,3-glucanase activity and strong binding to polymeric β-1,3-glucans.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".