Latent Herpes Simplex Virus Type 1 (HSV-1) in the Central Nervous System (CNS) of Immune Competant Mice Reactivates Spontaneously (P05.110)
Bibliographic record
Abstract
Objective: To determine if HSV-1 can spontaneously reactivate in the CNS of mice developing recurrent demyelinating lesions throughout the brain. Background We previously reported SJL/J, A/J, and PL/J mice develop lesions, characterized by demyelination, relative preservation of axons, and a mononuclear cell infiltrate, in the CNS following infection of the oral mucosa with HSV-1. In the early stage ( 3 months) of demyelination. Design/Methods: To determine if latent HSV-1 exists in the brains of A/J and PL/J mice, solution phase PCR was performed on [female] 10-12 week old mice infected with HSV-1 strain 2 via the oral mucosa. Immunohistochemistry (IHC) studies were also performed on the brains of these mice using α-HSV polyclonal antibody/ABC staining. Results: Using solution phase PCR, viral DNA was identified in the brains of both A/J and PL/J mice at 10 and 25 weeks PI. Further, a number of cells, with the morphology of neurons, stained positive with α-HSV antibody in the brainstems of A/J and PL/J but not C57BL/6 and Balb/c mice beyond 4 weeks PI. Conclusions: The results suggest that latent HSV-1 can reactivate spontaneously in the CNS of immune competent mice but is mouse strain dependent. The results raise the possibility that an immune response directed at reactivated virus could be responsible for the development of recurrent CNS demyelination in A/J and PL/J mice. Disclosure: Dr. Kastrukoff has nothing to disclose. Dr. Lau has nothing to disclose. Dr. Thomas has nothing to disclose.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".