Abstract 2123: Novel integration sites for Friend murine leukemia virus
Bibliographic record
Abstract
Abstract Background: Friend murine leukemia virus (F-MuLV), a type C retrovirus, induces erythroleukemia when injected into certain strains of mice. The Induction of erythroleukemia is associated with viral integration into Friend leukemia integration site-1 (Fli-1). In some F-MuLV-infected mice we did not observe viral integration into Fli-1 region by Southern blot analysis. We hypothesize that induction of leukemia in these mice is due to insertion of F-MuLV into a novel integration site distinct from Fli-1. Methods: The genomic DNA of F-MuLV-induced leukemic cells is digested by StuI restriction enzyme. DNA sequences of about 50 base pairs in length, termed adaptors, are then ligated to both ends of the digested fragments. Using primers for the adaptors and F-MuLV sequences, we performed PCR, followed by nested PCR to amplify those fragments that contain F-MuLV sequence. The PCR product is gel purified, cloned into a TA vector and sequenced. The sequences are compared to mouse genome databank to determine the F-MuLV flanking sequences. Results: We identified seven novel integration sites for F-MuLV. Three of these sites are located inside genes and the remaining four do not interrupt any genes. We performed PCR to check if any of these integration sites were also targets of F-MuLV in twenty other tumors of the same type. We found that one of these integration sites which is close to Myb gene occurred in twenty five percent of the tested tumors in an area 1 kb in length. Conclusion: We identified seven novel F-MuLV integration sites for F-MuLV. We showed that the integration site on chromosome 10 close to the Myb gene occurs in 25% of F-MuLV- induced tumors and is therefore a common integration site. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2123.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.003 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".