Single Nucleotide Polymorphism of Wx Gene Associated with Amylose Content in Barley Germplasm
Bibliographic record
Abstract
Objective Waxy gene (Wx in short) is a key gene responsible for the barley amylose synthesis.The objective of the present study was to detect single nucleotide polymorphisms (SNP) of Wx and determine the relationship between SNP and amylose content of seed in barley.Method After PCR amplification and gene clone,30 Chinese normal and waxy barley cultivars,which had high,medium and low amylose content respectively,were sequenced and polymorphism was analyzed for waxy gene with 2 Canadian waxy barley as controls.Result By polymorphism identification of waxy gene nucleotide sequences for the 32 barley cultivars,169 polymorphism sites were identified,which included 143 SNP and 26 InDel with frequencies of 1/310 and 1/196,respectively.It was found that the introns 1,3,5,8,exons 2,5 and 5'-UTR,3'-UTR were the rich nucleotide variation regions.Exon 2 and intron 1 suffered stronger selection pressure.Four haplotypes were identified from 32 plant materials.Among them,haplotype 1 included all low amylose content accessions.Conclusion The results of research showed that there is a clear correlation between SNPs in waxy gene and amylose content in barley.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".