Identification of ISSR Markers for Studying the Biodiversity of Bulgarian Representatives of Genus<i>Orobanche</i>Subsection<i>Minores</i>
Why this work is in the frame
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Bibliographic record
Abstract
The family Orobanchaceae consists of parasitic species. One group of taxonomically problematic species is subsection Minores (Beck) Teryokhin genus Orobanche. The species in this subsection (except O. crenata) have been often assigned to aggregate O. minor because of their great morphological similarity. The aim of this study was to find useful molecular makers for characterization of the biodiversity of subsection Minores, Orobanche in Bulgaria and their phylogenetic relationships.Six species of subsection Minores were examined: O. minor Sm.; O. amethystea Thuill.; O. esulae Pančić var. bulgarica T. Georgiev, O. pubescens d'Urv. O. loricata Rchb. and O. crenata Forssk. Genomic DNA was isolated from flowering stems and used as a template for ISSR PCR performed with ten different ISSR primers. The ISSR products were separated on agarose gel and visualized by UV-light. The molecular masses of the polymorphic bands were determined and used to fill Boolean matrices that were subjected to cluster analysis. The suitability of each primer for distinguishing each of the studied species was discussed. The resulting cladogram, based on the average Euclidean distances, displayed grouping by species and by geographic populations. This study confirmed that the assignment of the small-flowered species to agg. O. minor has only practical value and is not genetically justified. The study confirmed the independent taxonomic status of the Balkan endemic species O. esulae.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it