Abstract 2243: Prognostic significance of nuclear Sox4 expression in cutaneous melanoma and its role in cell migration
Bibliographic record
Abstract
Abstract Sry-related high-mobility group (Sox) family of genes is consisted of transcription factors involved in various physiological processes including embryonic development, cell fate decision and differentiation. Deregulated expression of Sox4 has been reported in several human cancer types so far; nevertheless the expression and possible role of Sox4 in human melanoma cancer progression is yet to be identified. To investigate the putative role of Sox4 in the development of melanoma and its correlation with patient survival, we examined the expression of Sox4 protein in different stages of melanocytic lesions and its role in melanoma cancer cells proliferation and migration. Using tissue microarray and immunohistochemistry, we evaluated Sox4 protein expression in 43 dysplastic nevi, 89 primary melanomas, and 48 metastatic melanomas. We observed a marked reduction in nuclear Sox4 expression in malignant melanomas compared with dysplastic nevi (Kruskal-Wallis test; p < 0.05) and primary melanomas (Kruskal-Wallis test; p < 0.01). Moreover, the reduced nuclear Sox4 expression was significantly correlated with a poorer disease-specific 5-year survival of patients with primary and metastatic melanoma (Kaplan-Meier test; P = 0.039). Multivariate Cox regression analysis revealed that reduced nuclear Sox4 expression is an independent prognostic factor to predict patient outcome (p = 0.049). We also knocked down the expression of Sox4 in MMRU human melanoma cell line by siRNA and the migratory ability of the cells as well as their proliferation rate were analyzed following Sox4 silencing. Strikingly, the wound healing assay revealed an augmented migration ability of MMRU cells upon siRNA mediated knockdown of Sox4. However, the absence of Sox4 did not affect the rate of melanoma cancer cells proliferation. Our results suggest that Sox4 may be a suitable marker for the prognosis of the melanoma cancer outcome and a potential therapeutic target for human melanoma. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2243.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".