PWE-076 Dendritic Cell Characteristics in Pouchitis
Bibliographic record
Abstract
Introduction The role of dendritic cells (DC) in inflammatory bowel disease is increasingly recognised for their function in regulating intestinal immune responses. To our knowledge there are no previous studies of DC in pouchitis. We aimed to characterise changes in DC in pouchitis that may underlie the dysregulated immune response to the pouch microbiota. Methods Mucosal biopsy samples were taken from patients with pouchitis (n = 14) and ulcerative colitis patients without pouchitis (n = 10). Lamina propria DC were isolated by collagenase digestion. DC were identified as an HLA DR+, lineage -(CD3-, CD14-, CD16-, CD19-, CD34-) population. DC expression of TLR 2 and 4, CCR9, β7 and CD40 were measured by multicolour flow cytometry. The t-test was used for statistical analysis. Results DC expression of TLR 2 and 4 were both significantly elevated in patients with pouchitis compared with non-pouchitis patients (p = 0.007 and 0.008). In pouchitis patients, DC expression of β7 was increased (p = 0.02) and expression of CCR 9 was decreased (p = 0.02). DC expression of CD40 was increased in patients with pouchitis (p ≤ 0.0001). Conclusion In pouchitis, DC are activated and upregulate expression of microbial recognition receptors. In addition, DC expression of gut homing markers is elevated in pouchitis with a more colonic homing marker profile. Similarly to other IBD, DC are likely to be key in the initation and perpetuation of the inflammatory response to the dysbiosis of the pouch microbiota Disclosure of Interest J. Landy Grant/Research Support from: The Broad Foundation, H. Al-Hassi: None Declared, E. Mann: None Declared, S. Peake: None Declared, P. Ciclitira: None Declared, J. Nicholls: None Declared, S. Clark: None Declared, S. Knight: None Declared, A. Hart: None Declared
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".