Fatty Acid Transport Proteins Translocate To The Muscle Membrane During Exercise Before And Following Training
Bibliographic record
Abstract
Previous work demonstrated that the content of plasma membrane fatty acid translocase (FAT/CD36) protein was acutely increased (translocation) during prolonged moderate intensity exercise, during which whole body fat oxidation increased 2-fold in a group of untrained men and women. However, there was no increase in plasma membrane fatty acid binding protein (FABPpm) during the exercise. In addition, 6 weeks of high-intensity interval training (HIIT) increased the plasma membrane content of FABPpm, but not FAT/CD36 in women. PURPOSE. This study examined the response of plasma membrane FAT/CD36 and FABPpm during 2 hr of acute moderate intensity exercise, both prior to, and following 6 weeks of HIIT in male subjects. METHODS. Ten recreationally active men trained 3 days/week for 6 weeks (18 sessions) on a cycle ergometer. Training consisted of 10 × 4 min bouts at ∼90% VO2 peak, separated by 2 min of rest (HIIT). Training power outputs were increased to maintain heart rate at ∼90% of maximum. A VO2 peak test was conducted pre and post training, as well as a 2 h ride at ∼65% of pre-training VO2 peak. Two skeletal muscle biopsies were taken at rest and again following 2 hr of cycling and expired gases were sampled throughout exercise. Giant sarcolemmal (plasma membrane) vesicles were prepared from the muscle biopsies. RESULTS. VO2 peak significantly increased by ∼20% (3.8 ± 2 to 4.6 ± 2 L/min) following training and whole body fat oxidation increased during the 2 hr of exercise (pre-training: 0.4 ± 0.1 to 0.9 ± 0.1 g/min; post-training: 0.6 ± 0.1 to 1.0 ± 0.1 g/min). The acute exercise bout prior to training increased plasma membrane FAT/CD36 (40%, n = 10) and FABPpm (30%, n = 9). Following 6 weeks of HIIT, the transport proteins were again acutely increased by prolonged exercise at the same pre-training intensity (FAT/CD36, 32%, n=6; FABPpm, 25%, n = 6)). CONCLUSIONS. Plasma membrane FAT/CD36 and FABPpm content were increased with acute moderate intensity exercise in men before and after 6 wk of HIIT. We believe the increases in plasma membrane fat transport proteins are important for increasing plasma free fatty acid transport into skeletal muscle and ultimately increasing oxidation during prolonged moderate intensity exercise. Funded by CIHR and NSERC, Canada.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".