ALDH1 is a useful marker of basalness in human breast cancer and its stem/progenitor cells.
Bibliographic record
Abstract
Abstract Abstract #104 Background: The basal cell subtype is a particularly aggressive form of breast cancer. The molecular mechanisms which underlie the aggressive course of basal cell breast cancer, as well as its corresponding tumor markers are currently under intense study. In addition, there is increasing evidence that cancer stem/progenitor cells are integral to the formation and perpetuation of breast cancer. Together with their progenitors, cancer stem cells may determine the therapeutic outcome and the clinical course of cancer. The first isoform of aldehyde dehydrogenase (ALDH1) has recently been reported to be a marker of breast stem/progenitor cells. Thus, we undertook this study to determine whether the immunohistochemical detection of ALDH1 in fixed breast cancer tissues correlates with selected basal and luminal cell surface markers. Methods: Formalin-fixed, paraffin-embedded specimens were studied in triplicate using tissue microarrays from 57 breast cancer patients. In addition to ALDH1 staining, each of the following markers were studied using immunohistochemistry (IHC) in each sample: ER, PR, HER-2, the basal cell markers EGFR/HER1, CK5, CK14, CK17, SMA, and the luminal cell markers CK19, and EMA. Results: ALDH1 staining was performed using IHC and subsequently evaluated using the Quick score. The Quick score takes into account both the percentage of positive cells, as well as the corresponding staining intensity. Our results suggest that breast cancer samples expressing the triple negative phenotype (ER-, PR-, HER-2 negative) together with one or more basal cell markers (EGFR/HER1,CK5, CK14, CK17, SMA) are associated with an elevated Quick score for ALDH1 staining, when compared with samples lacking the expression of these markers. Conclusions: As it has been demonstrated that IHC detection of ALDH1 detects stem/progenitor cells in fixed breast samples, our results suggest that an elevated Quick score is associated with the enrichment of IHC basal cell markers in these samples. We are currently focusing on a larger number of breast cancer samples so that correlations can be studied as a function of therapeutic outcome and overall survival of breast cancer patients. Citation Information: Cancer Res 2009;69(2 Suppl):Abstract nr 104.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.005 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".