Heat shock protein 70 can interact with and also prevent the high molecular weight FGF2‐induced apoptotic chromatin compaction
Bibliographic record
Abstract
Fibroblast growth factor 2 (FGF2) is translated as high molecular weight (hi) and low molecular weight (lo) isoforms. We reported that increased nuclear expression of hi but not lo FGF2 elicited chromatin compaction and apoptotic cell death. We have begun investigating the role of hi FGF2 isoform‐specific interacting partners in modulating its pro‐apoptotic effect. Isoform‐specific affinity chromatography was used to capture interacting proteins from cell cytosolic and nuclear fractions. Nuclear proteins that bound only to the hi FGF2‐affinity column were identified by MS/MS peptide sequencing, and included the heat shock protein 70 (hsp70). Hsp70 was confirmed as a hi (but not lo) FGF2 interacting partner by co‐immunoprecipitation experiments. Overexpession of hsp70 prevented the hi FGF2‐induced chromatin compaction in cardiomyocytes. While hsp70 was mainly cytosolic in the absence of over‐expressed hi FGF2, it translocated to the nucleus in the presence of hi FGF2 over‐expression. The nuclear pattern of hsp70 localization partially overlapped with that of hi FGF2, suggesting interaction in situ. In conclusion, hsp70 interacts with hi but not lo FGF2, and is capable of preventing nuclear compaction by hi FGF2. It is possible that an in situ interaction between hsp70 and hi FGF2 is preventing the latter from exerting its apoptotic chromatin compaction effects.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".