Structure of the tetracycline-degrading monooxygenase TetX2
Bibliographic record
Abstract
Page s144 s144The toxic extracellular endopeptidase AsaP1 is the causative agent of Aeromonas salmonicida achromogenes and leads to atypical furunculosis, a systematic disease in Atlantic salmon and other farmed fish (1).AsaP1 is 343 amino acids long zinc-metallopeptidase containing a signal sequence of 22 amino acids and an Nterminal propeptide of 171 amino acids, which is released by autocleavege leading to an active enzyme of 22 kDa.For biochemical and structural characterisation an E. coli expression system was established and the structure elucidation for the two inactive mutants AsaP1_E294Q and AsaP1_E294A was performed by X-Ray crystallography.The structure was solved by molecular replacement using the search model of the peptidyl-Lys metalloendopeptidase from Grifola frondosa composed of a 172 amino acid protease domain without propeptide (pdb entry 1g12).Due to inactivity of AsaP1 mutants the propeptide remains still as a part of the protein, but its function, whether it plays a role in protease folding and acts as an intramolecular chaperone or whether it inhibits proteinase activity in the intracellular space, still remains unclear.The complex build of propeptide domain and protease domain reveals insights for substrate interaction and substrate specificity of AsaP1.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.007 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".