Proximate composition analysis posterior to the cryopreservation of Chaetoceros calcitrans
Bibliographic record
Abstract
ABSTRACT Objective. The effect of cryopreservation on the proximate composition of microalgae Chaetoceros calcitrans was evaluated. Materials and methods. C. calcitrans was cultured and cryopreserved using 5% (v/v) dimethylsulfoxide as cryoprotectant. The freezing was controlled at a rate of 3°C/min, up to -60°C and then the microalgae were immersed in liquid nitrogen (-196°C). After storage in liquid nitrogen, microalgae were rapidly thawed out and subcultured. The percentage of proteins, lipids and carbohydrates was analyzed using absorption spectrophotometry and the organic matter was studied by gravimetric analysis. Results. There was no significant variation between the proximate composition of C. calcitrans cryopreserved and the controls (p>0.05). Conclusion. Our results show that, despite low cell recovery after the preservation of this organism at low temperatures, there is no apparent loss of nutritional characteristics caused by the storing process at low temperatures. RESUMEN Objetivo. En este estudio se evaluó el efecto de la técnica de criopreservación sobre la composición proximal de la microalga Chaetoceros calcitrans. Materiales y métodos. C. calcitrans fue cultivada y criopreservada empleando dimetilsulfóxido al 5% (v/v) como crioprotector. La congelación fue controlada a una tasa de 3°C/min, hasta llegar a -60°C y luego las microalgas fueron sumergidas en nitrógeno líquido (-196°C). Posteriormente, las microalgas fueron descongeladas de manera rápida y nuevamente cultivadas. Seguidamente se analizó la cantidad porcentual de proteínas, lípidos y carbohidratos, empleando espectrofotometría de absorción y la materia orgánica por gravimetría. Resultados. Los resultados indicaron que la composición proximal posterior a la criopreservación no evidencia diferencias significativas respecto a los controles no criopreservados (p>0.05). Conclusiones. Los resultados indican que pese a la baja viabilidad post-criopreservación de C. calcitrans, no hay pérdida aparente de la composición nutricional debida al almacenamiento a bajas temperaturas.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".