Isolation and identification of feruloylated arabinoxylan mono- and oligosaccharides from undigested and digested maize and wheat
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Feruloylated arabinoxylan mono- and oligosaccharides (F-AXOS) are a subject of interest because of their prebiotic and antioxidant properties. We aimed at isolating and identifying F-AXOS from maize, wheat, wheat bran and wheat aleurone using HPLC and LC-MS/MS. Prior to extraction of F-AXOS, samples were subjected to either simulated gastric fluid with enzymes (gastric) or without enzymes (pH) or water (aqueous) at 37 °C. F-AXOS present in all samples were identified as 5-O-feruloyl-α-L- arabinofuranose and possibly 5-O-feruloyl-α-L-arabinofuranosyl-(1 → 3)-O-β-D-xylopyranose. Their mean content, measured as esterified ferulic acid (FA), was 2.5 times higher in maize (10.33 ± 2.40 μg/g) compared to wheat. Digestion under gastric or pH conditions resulted in a two-fold increase in F-AXOS in all samples. The level of F-AXOS produced during gastric or pH condition was positively correlated to the insoluble bound FA content of the sample (R(2) = 0.98). 5-O-Feruloyl-α-L- arabinofuranose was the only identifiable F-AXOS released during gastric digestion. Our results suggest feruloyl arabinose is the most abundant form of F-AXOS in maize and wheat.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it