Vascular endothelial cell growth factor-C small RNA interfering vector down-regulate COX-2, Bel-2 and induce apoptosis in human breast cancer cells
Bibliographic record
Abstract
Objective To clone the recombinant plasmid silencing vascular endothelial growth factor-C (VEGF-C) gene by small interfering RNA (siRNA) and observe its role in the expression of COX-2,Bcl-2 and proliferation in human breast cancer cells MDA-MB-435. Methods Breast cancer cells MDA-MBA-435 were transfected stably with the recombinant siRNA plasmid and negative control by positive liposomal methods.We examined the gene expression of VEGF-C,COX-2 by RT-PCR and protein expression of VEGF-C,COX-2 and Bcl-2 by Western blot to explore the effect of VEGF-C down-regulation.The influences on cell activity and apoptosis were studied by MTT and flow cytometry. Results The down-regulation of VEGF-C inhibited the expression of some other genes such as COX-2 and bcl-2.The apoptosis rate was 60% after transfection 72 hours,and grew much slower than control groups. Conclusions The results obtained explain the function of VEGF-C and the cross-talk of downstream tyrosine kinase.Silencing of VEGF-C expression using siRNA vectors inhibits cell activity and induces apoptosis in human breast cancer cells MDA-MBA-435,which is a potential approach for tumor gene therapy.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".