Design of SSR Primers and Verification of E-PCR in Non-coding Regions of Sorghum Genome
Bibliographic record
Abstract
The complete genome sequence of sorghum which downloaded from the NCBI public databases was used for a simple sequence repeated SSR search.The result showed that there were 87950 SSR loci and 1134 kinds of repetition about the results in all.The proportion of dinucleotide and trinucleotide repeats was placed on the first place,accounting for 43.36% and 32.38% of the all.SSR primers of sorghum were designed basing on the search results of SSR,according to use the e-PCR to validate and screen primers two times,50 pairs of primers were arbitrarily selected and synthesized to evaluate these primers by sorghum-sudangrass hybrids such as Xian-Feng and Le-Shi.The results showed that there were 46 target bands among 50 pair primer,and the target bands were characterized clearly and steadily,of which only 8 pairs had polymorphism.It indicated that the polymorphism of sorghum designed primers in non-coding region was not higher than the EST-SSR primers,but it played a complementary and catalytic role on the development of SSR primers in sorghum.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".