Molecular Cloning and Analysis of the N4 Neuraminidase Subtype from an Avian Influenza Virus
Bibliographic record
Abstract
Avian influenza viruses(AIV) are divided into different subtypes based on the antigenicity of the surface protein hemagglutinin(HA) and Neuraminidase(NA),and 15 HA subtypes and 9 NA subtypes have been isolated from birds.The influenza viruses with N1(H1N1,H5N1) or N2(H2N2,H3N2,H5N2 and H9N2) are widely circulated in birds,pigs and humans,and influenza viruses with N6(H4N6) and N8(H3N8) are circulated in pigs and horses,respectively,however,the influenza viruses with other NA subtypes are only seldom isolated from wild birds,and the sequence information of these NAs are very limited in the GenBank.In this study,the NA gene of N4 subtype influenza virus A/Turkey/Ontario/6118/68(H8N4) was amplified,cloned and sequenced.Results showed that the N4 NA gene has one open reading frame and encodes 470 amino acids.Deduced amino acids sequence showed that there are 9 potential glycosylation sites and 20 cysteines in the N4 NA protein.The sequence in this study is the first report of the full length N4 NA gene,and it will provide the necessary information for the further analysis of the structure and function of N4 NA of AIV.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".