Further characterization of the large subunit of the major embryonic Artemia franciscana cysteine protease.
Bibliographic record
Abstract
The encysted embryos of Artemia franciscana contain a potentially unique cysteine protease consisting of a cathepsin L-like protease and a tightly associated protein which has been denoted as the large subunit. Whereas the small subunit has been characterized in this laboratory as a cathepsin L-like cysteine protease, the large subunit has not been studied. The dimeric cysteine protease from Artemia encysted embryos was purified to apparent homogeneity using a multi-step method involving gel filtration, anion exchange and affinity chromatography. Five isoforms were separated by fast protein liquid chromatography. The protease subunits from each isoform were isolated using reverse phase high performance liquid chromatography. Protease Lys C and cyanogen bromide treatments were used to generate fragments for N-terminal amino acid sequencing of the large subunit from the major isoform of Artemia cysteine protease. Chromatography of the Artemia cysteine protease on Mono S at pH 5.0 led to the purification of the subunits in the native state. Based on molecular and biochemical data, several possible roles for the large subunit of Artemia cysteine protease are proposed in this thesis. (Abstract shortened by UMI.)Dept. of Biological Sciences. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1999 .P85. Source: Masters Abstracts International, Volume: 39-02, page: 0453. Adviser: Alden Warner. Thesis (M.Sc.)--University of Windsor (Canada), 2000.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".