Bibliographic record
Abstract
Using a wheat chitinase gene fragment derived from PCR as probe, a Chid1 chitinase gene, Wch2 , was isolated from a wheat genomic library. Sequence analysis indicated that Wch2 encodes a peptide of 311 amino acids with no intron, which contains a signal peptide, a cysteine-rich chitin-binding domain, two variable regions and two catalytic domains (Fig.1). Southern blot hybridization showed that there are multiple copies of Wch2 in wheat genome (Fig.2). Infection with stem rust fungus Puccinia graminis induced a differential expression of Wch2 in a pair of near-isogenic wheat lines (Fig.3). In resistant isogenic line Chinese Spring Sr11(CS-Sr11), an induction of Wch2 transcript was detected 3 days after inoculation and a massive increase of the expression was observed 6 days after incolation. In contrast, in susceptible line Chinese Spring sr11(CS-sr11), expression of the chitinase gene was not detected during the time courses assayed. Crude bacterial extracts of Wch2 expressed in E.coli showed a clear chitinase activity towards chitin(Fig.4). These results suggest that Wch2 may play an important role in the induced resistance responses to the stem rust fungus.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".