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Record W2354786892

Cloning and Sequence Analysis of c-Myc Gene in Goat

2014· article· en· W2354786892 on OpenAlexaff
AN Chen-ru

Bibliographic record

VenueChina Animal Husbandry & Veterinary Medicine · 2014
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicAnimal Genetics and Reproduction
Canadian institutionsScience North
Fundersnot available
KeywordsGenBankGeneHomology (biology)BiologySequence analysisCoding regionGeneticsMolecular biologyNucleic acid sequencePeptide sequence
DOInot available

Abstract

fetched live from OpenAlex

In order to clone sequence of the coding region of the goat c-Myc gene,this study designed specific primers by refering to c-Myc genes coding sequence reported in GenBank including sheep,cattle,pigs,horses,etc.,and then amplified c-Myc coding sequence from embryonic skin tissue of goats by using RT-PCR technique and had sequence analysis.The result indicated that CDS of goat c-Myc gene had 1320bp length including the stop codon.For only 10differences comparing to sheep c-Myc gene nucleotide sequence(GenBank accession number:Z68501.1),homology reached 99.17% and homology of deduced amino acid sequence reached 99.09%.Analysis of homology with other species showed that homology of goat c-Myc gene coding region nucleotide sequence was 91.2%,92.0%,90.2%,86.9%,86.0% with pigs,wolves,humans,rats,mice,and homology of deduced amino acid sequence was 93.6%,96.1%,92.3%,91.1%,89.6%.Using bioinformatics software analysis showed that goat c-Myc protein was localized in the nucleus and contained a HLH domain.This study further researched the function of c-Myc gene and laid the foundation of investigating the effect in the process of goat somatic cell reprogramming.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.874
Threshold uncertainty score0.512

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.025
GPT teacher head0.297
Teacher spread0.272 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2014
Admission routes1
Has abstractyes

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