Molecular Cloning and Sequence Analysis of DRM Gene Segment of Fraxinus mandschurica
Bibliographic record
Abstract
[Objective]The aim was to clone and analyze the domain remethylation enzyme(DRM) gene fragment of Fraxinus mandshurica.[Method]According to the sequence of DRM amino acid published in GenBank,a pair of degenerate primers was designed and synthesized by the CODEHOP method.DRM gene of F.mandshurica was amplified by RT-PCR technique.The PCR product was connected with pMD18- T and transformed into competent cells of E.coli JM109.Then the positive clone was identified and the sequence was sequenced and analyze.[Result]The cDNA sequences of cloned DRM gene with named FmadrmA was 802 bp in length,and coded 267 amino acid residues.Sequence alignment showed that FmadrmA presented high homology with other 12 species' DRM at the level of amino acid.Compared with the corresponding region of grape,tomato,cucumber,wild strawberry,soybean,alfalfa,corn,tobacco,Arabidopsis,poplar,rice and wheat,the protein homology of F.mandshurica was 61%,54%,51%,50%,60%,48%,43%,44%,37%,42%,42% and 39%,respectively.Phylogenetic tree analysis indicated that the DRM proteins with homology could be roughly classified into three categories,and F.mandshurica had a far genetic relation with other palnts.[Conclusion]The FmadrmA gene fragment of F.mandshurica was obtained successfully,and the homology was analyzed.It laid the foundation for the further research on domain remethylase gene and analysis of its expression characteristics.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".