Expression and immunogenicity study of virus like particles of eastern equine encephalitis virus
Bibliographic record
Abstract
This study designed to explore the immunogenicity of virus like particles(VLPs) of eastern equine encephalitis virus(EEEV). All encoding protein genes, C-E3-E2-6k-E1 of EEEV, were amplified using PCR technique. The PCR products were digested and inserted into pFastBacTM1 plasmid, designated as pFastBacTM1-C-E. After identification, the pFastBacTM1-C-E plasmid was recombinated with BmNPV(Bombyx mori nuclear polyhedrosis virus) by genetic recombining method to construct BmNPV-C-E, which was responsible for the production of the recombinant baculovirus. EEEV VLPs were expressed by BmNPV-C-Es transfected BmN cells. After VLPs were detected using the electron microscopy and indirect immunofluorescence(IIF) assay, they were purified by discontinuous sucrose gradient method. The Balb/c mouse was intramuscularly inoculated twice with a dose of 15 μg VLPs plus freund's adjuvant at an interval of 14 days in the right and left quadriceps. Seven days after the boost immunization, the ratio of CD4+T cell/CD8+T cell and the concentrations of IL-2, IFN-γ and TNF-α in peripheral blood of mice were detected; fourteen days after the boost immunization, the titers of IgG antibody were evaluated using ELISA assay. The data showed the ratio of CD4+T cell/CD8+T cell and the concentrations of IL-2, IFN-γ and TNF-α of experimental group were significant difference compared with the control groups in the whole experiment(P 0.01), and the titers of IgG antibody of experimental group and VLP group were 1∶ 128 and 1 ∶64, respectively, however, no IgG antibody was detected in samples from the control groups. Therefore, EEEV VLPs generated by the baculovirus expression vector system(BEVS) exhibit strong immunogenicity in mice, which could provide a novel,safe, non-replicating and effective vaccine candidate against EEEV infections.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".