Construction and Identification of Recombinant Pseudovirus Carrying N-linked Glycosylation-site Mutants of Hantaanvirus
Bibliographic record
Abstract
Objective: To further study the effect of hantavirus envelope glycoprotein glycosylation on viral immunogenicity and infectious, five recombinant pseudovirus carrying N-linked glycosylation site mutants of Hantaanvirus(HTNV) were constructed.Methods: Mutated five N-glycosylation sites of GP in HTNV 76-118 strain using site-directed mutagenesis methods and constructed recombinant pseudovirus by cotransfecting the Lentiviral expression vector and packaging vectors into the HEK293 cells. Then the five recombinant pseudovirus were identified by RT-PCR and Immunofluorescence(IFA). Results: Five N-linked glycosylation site mutants were contrasted and their sequencing showed the asparagines(N) residues at the N-linked glycosylation sites on Gn and Gc were replaced by glutamine(Q). RT-PCR results showed that all of the five recombinant pseudovirus could express the HTNV GP gene. IFA results showed that the recombinant pseudovirus also could express the Gn and Gc protein of HTNV. Conclusion: The five recombinant pseudovirus carrying N-linked glycosylation site mutants of HTNV were successfully constructed and named rLV-M1, rLV-M2, rLV-M3,rLV-M4 and rLV-M5, respectively. The result of this study provided tools for study on the roles of N-linked glycosylation of HTNV glycoproteins in biological activity and laid the foundation for futher esearch of hantavirus vaccine and pathogenic mechanism.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".