In Silico Cloning,Bioinformatics and Adaptive Evolution Analysis of rbcL Gene in Chrysanthemum morifolium
Bibliographic record
Abstract
Objective:Ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)from Chrysanthemum morifolium was cloned in silico cloning.rbcLgene sequences in silico cloning,properties of encoding amino acid and adaptive evolution were analyzed.Method:According to the related literature,a full-length rbcLgene sequences form Chrysanthemum morifolium was cloned in silico cloning,and the structure and function of rbcLprotein were predicted by bioinformatics analysis.In the site-specific model,we found amino acid positive selection sites inrbcLgene.Result:A complete open reading frame sequences of electronic cloning chrysanthemumrbcLgene was 1 452 bp and encoded 483 amino acids.rbcLprotein secondary structure has 19α-helices and 18β-sheets.Seven amino acid sites(228S,255 V,279T,309 M,328S,439 T,449T)were found under positive selection.Conlusion:The complete sequence fromChrysanthemum morifolium rbcLgene in silico cloning provided certain reference data for further study,and encoded protein adaptability research was helpful to explore the mechanism of compositae adapting to the environment.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".