Lytic Replication and Inductive Production of Recombinant Epstein Barr Virus Visualized
Bibliographic record
Abstract
In order to study single viral gene functions in the context of genome and analysis interactions between one gene with another, the HEK293/p2089 stable cell line was established by transfecting the plasmid of DNA (p2089) into EBV-negative 293 cells and selecting for resistance against hygromycin. The plasmid p2089, which was kindly provided by Prof.Hammerschmidt, contained the whole EBV genome of wild-type B95-8. Two eukaryotic expression vectors (pcDNA3.1(+)/BZLF1 and pcDNA3.1(+)/BALF4) were constructed and then transiently cotransfected into the HEK293/p2089 stable cells, so as to induct EBV lytic replication and product recombinant EBV particles visualized through GFP-expressing. To estimate the EBV production, Raji cells were incubated with supernatants from the induced 293 cells carrying p2089 DNA, as revealed by indirect visualization of the Raji cells. GFP-positive cells were evaluated by inverted fluorescence microscope or FACS analysis. The different virus supernatants were quantified with the help of green Raji units per ml as an absolute number of infectious particles. This technique makes it possible for the reconstitution of viral progeny or mutants by transfection of BAC plasmid into eukaryotic cells, and any genetic modification in E. coli, thereby facilitating the analysis of viral gene functions in the context of genome. This new technique has provided a useful tool for the study of pathogenesis mechanism of EBV, especially for that of cancer-associated.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.002 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".