Isolation and genomic sequence analysis of porcine circovirus type 2 from pigs
Bibliographic record
Abstract
Post-weaning multisystemie wasting syndrome (PMWS) is an emerging disease in swine. Porcine circovirus type 2 (PCV2) has been associated with the PMWS. Two PCV2 strains were isolated from pigs showing signs of PMWS in Fujian Province. They were denominated FUQING0401 and PUTIAN0401. the sequencing of 2 PCV2 strains along with the phylogenetie analysis of these strains and those of 12 PCV2 sequences published in GenBank were compared. Sequence analysis of the complete genome indicated that the Fujian PCV2 strains were closely related to each other but also to other PCV2 strains originating from Canada, USA, Europe and China. Sequence analysis of ORF1 genes of the 2 strains revealed that the extent of nucleotide homologous was 97.5 %. The amino acid sequences alignment of the PCV2 replicatase protein identified 6 amino acid heterogeneity. Capsid protein genes of PUTIAN0401 and FUQING0401 homologous rate was 99.7%, and the amino acid homologous rate was 98.7%. Phylogenetic analysis revealed that the 14 strains could be divided into several clusters, but the homologous rate was different from 91.6%to 99.9%. The two strains from Fujian were spread in small groupings and an association with geographic origin could not be established.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".