Effect of Tetramethylpyrazine in combination with cisplatin on the growth and angiogenesis of transplanted Lewis lung carcinoma in mice
Bibliographic record
Abstract
Objective To investigate the inhibitory effect of Tetramethylpyrazine( TMP) in combination with cisplatin on the tumor growth and angiogenesis in mouse Lewis lung cancer. Methods Lewis lung carcinoma cells were inoculated subcutaneously into right axillary of C57B /6 mice,and the mice were randomly divided into 4 groups: model group,TMP group,cisplatin( DDP) group and combination group( TMP+ DDP). From day 7 after inoculation,all the mice were given different treatments for 14 days,and change of the xenografted tumor volumes was monitored. All mice were sacrificed on day 21. Subcutaneous tumors were processed for tumor weight,and tumor growth inhibition rate and tumor necrosis rate of each group were calculated. The expression level of Kruppel-like factor 4( KLF4), vascular endothelial growth factor( VEGF) were measured by Western blot. The microvascular density( MVD) was determined by CD105 staining. Results The tumor growth was suppressed in TMP group,cisplatin group and combination group.The weights of tumor were markedly decreased in TMP group,cisplatin group and combination group compared with model group( P 0. 05). The combination group showed significant enhancement in anti-tumor efficacy than TMP group and DDP group( 62. 48% vs 35. 58% vs 40. 41%,P 0. 01). Compared with DDP group,the tumor necrosis rate in combination groupwas significantly higher [( 24. 64 ± 11. 96) % vs( 31. 76 ± 15. 20) %,P 0. 05],but not remarkably in TMP group [( 15. 58 ± 3. 19) %,P 0. 05]. The expression levels of vascular endothelialgrowth factor and microvessel density were significantly lower,Kruppel-like factor 4 was significantly higher in TMP group,cisplatin group and combination group than that in model group( P 0. 05). Meanwhile,there was no significant difference between cisplatin group and tetramethylpyrazine group( P 0. 05). Conclusion TMP has synergetic inhibitory effect with DDP on the growth and angiogenesis of Lewis lung carcinoma effectively by inhibiting expression of VEGF,enhancing expression of KLF4.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.003 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".