Cloning and sequencing of OsMAPK4 gene and construction of its plant expression vector
Bibliographic record
Abstract
A 1 500 bp DNA fragment amplified by RT-PCR use OsMAPK4 gene special primer from liaoyan241 leaf treated by low temperature was cloned into pUC18. The result of sequencing showed that the sequence of this fragment was 99.4% homologous with that of OsMAPK4 gene in GenBank and amino acid homologous is 99.1%. Further more OsMAPK4 gene was cloned into plant express vector cassette pBCE12 and pBC29A. Thus two plant express vectors, pBME12 and pBM29A were constructed, in which OsMAPK4 gene was controlled by E12 promoter and rd29A promoter respectively. Then the recombination plasmids were introduced into Agrobacterium LBA4404 by freezing-melting transformation method. This work provides a foundation for transferring OsMAPK4 gene into plant by Agrobacterium-mediated method and its function analysis.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".