Establishment of Reverse Transcription Helicase-Dependent Isothermal Amplification for Rapid Detection of Foot-and-Mouth Disease Virus
Bibliographic record
Abstract
A rapid detection method of foot- and- mouth disease virus( FMDV) was established based on reverse transcription helicase- dependent isothermal amplification( RT- HAD). Its sensitivity and specificity were assessed and compared with RT- PCR method. The results showed that target fragment of FMDV RNA could be amplified by incubating at 65 ℃ for 120 minutes using a pair of primers designed based on the conservative sequence of foot- and- mouth disease virus genome. The detection limit of this method was 0. 2 ng of RNA sample which was 10 fold higher than that of RT- PCR.The specificity of this method is also high. We couldn't detection swine vesicular disease virus( SVDV) 、porcine reproductive and respiratory syndrome virus( PRRSV) 、swine fever virus( CSFV) 、porcine parvo virus( PPV) and vesicular stomatitis virus( VSV) by this method with the primers of FMDV.RT- HDA detection method of FMDV not only has high sensitivity and specificity,but also does not require expensive equipment. These properties offer a great potential for the development of simple portable DNA diagnostic devices to be used in the field and at the point- of- care.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".