Studies on the Interaction between Daidzein and Bovine Serum Albumin by Fluorescence Spectroscopy
Bibliographic record
Abstract
The interaction between Daidzein and bovine serum albumin(BSA) in physiological buffer(pH=7.4) was studied by fluorescence spectroscopy.The quenching constants were obtained at 290 K,303 K,310 K and 315 K,which decreased with increase of the temperature and all the quenching constants were larger than 2×1010L·mol-1·s-1(the maximum collision quenching constant of various quenchers).The results indicated that the fluorescence quenching mechanism for BSA through Daidzein binding was likely a static quenching process.According to the Frster theory of non-radiation energy transfer,the binding distance(r) was calculated as 4.02 nm.The thermodynamic parameters,enthalpy change(△H) and entropy change(△S),were calculated to be-238.213 kJ·mol-10,and-711.855 J·mol-1·K-10,respectively,which indicated that the interaction of Daidzein with BSA was driven mainly by the hydrogen bond and Van der Waals force.The process of binding was a spontaneous process in which Gibbs free energy changes were negative(△rGm=-17.538 kJ·mol-1(310 K),△rGm=-13.978 kJ·mol-1(315 K)).The effect of Daidzein on the conformation of BSA was analyzed using synchronous fluorescence spectroscopy.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".