Development and Application of Molecular Markers Specific to Chromosome 6VS of Haynaldia villosa
Bibliographic record
Abstract
The resistance gene of powdery mildew was introduced from Haynaldia villosa into common wheat(Triticum aestivum) and located on 6VS and designated as Pm21.In order to precisely map and clone Pm21,more molecular markers and chromosome structural variants need to be developed.RGA and STS markers,usually from expressed sequences,have proved to be very effective DNA markers.In order to development and application of molecular markers specific to chromosome 6VS of H.villosa,the DNA polymorphic analysis was performed on common wheat Yangmai 5,H.villosa and wheat-H.villosa 6VS/6AL translocation lines with 11 RGA and 17 pairs of STS primers.Two polymorphic fragments,about 1 000 bp and 800 bp,were amplified by a RGA primer Pto kin4 and a pair of STS primer,and then were cloned and sequenced,respectively.Based on the cloning sequences,two pairs of specific markers,CINAU17-1086 and CINAU18-723,were developed,and used to amplify common wheat Chinese Spring,Yangmai 5,Yangmai 158,H.villosa and T.durum-H.villosa amphiploid,wheat-H.villosa alien addition lines(1V-7V),wheat-H.villosa 6VS/6AL translocation line 92R137.The results indicated that two markers,CINAU17-1086 and CINAU18-723,were located on chromosome 6VS because only the materials with 6VS chromosome could amplify the specific patterns.Furthermore,CINAU17-1086 was located between FL0.58 and FL0.70 and CINAU18-723 between FL0.45 and centromere on chromosome 6VS by using 6VS deletion addition lines and translocation lines.The results obtained from these two markers were consistent with those from the cytogenetic identification to variant materials of chromosome 6VS obtained by radiating pollen.Therefore,CINAU17-1086 and CINAU18-723 markers could be used to rapidly detect the correspondent chromosome segments of 6V in common wheat and primarily identify the breakage points of deletion lines.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".