Analysis of differentially expressed transcripts in early and middle development of Betula platyphylla female inflorescence
Bibliographic record
Abstract
Betula platyphylla is a monoecious tree species with unisexual flowers,which plays an important role in the study of floral development of woody plants. This study aims to initially identify gene expression and regulation during the critical periods of female flower development in B. platyphylla,megasporogenesis( prophase) and gametophyte development( metaphase),and to discover genes related to female floral development. Firstly,we ascertained the prophase and metaphase in female floral development by the cytological method. Secondly,the establishment of forward and reverse suppression subtractive hybridization( SSH) c DNA libraries BHHQ and BHHZ were carried out with the c DNA of prophase and metaphase female inflorescence as tester and driver respectively,using SSH technique. A total of 1406 high quality EST sequences were obtained,787 of them having high homologies with the nucleic acid or protein sequences in databases and 47 genes possibly associating with flower development.The results of GO analysis showed that 34. 96% of these genes are mainly involved in biosynthesis process,16. 45% in cellular component and 48. 59% in molecular function. Six flower developmentrelated genes were analyzed by qRT-PCR analysis and the results showed that each of these genes has some expression in different tissues. Among them,the expression level of bhhq. 408,bhhq. 435,bhhz.359,and bhhz. 215 in reproductive organs was significantly higher than that in other organs,respectively;both bhhq. 254 and bhhq. 190 have some expression in most tissues,confirming that these genes are notonly involved in the development of reproductive organs,but also play some roles in vegetative organs.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".