Study on the Interaction Between Luteolin and Bovine Serum Albumin by Fluorescence Spectroscopy
Bibliographic record
Abstract
The interaction behavior between luteolin and bovine serum albumin(BSA) in physiological buffer (pH=7.4) was studied by fluorescence spectroscopy. The quenching constants were obtained at 292 K,299 K and 311 K. The results indicated that the fluorescence quenching mechanism for BSA through luteolin binding was a static quenching process. The thermodynamic parameters,enthalpy change (△H) and entropy change (△S),were calculated to be-4.755 kJ\5moL-1 and 93.2 J\5moL-1\5K-1 respectively,which indicated that the interaction of luteolin with BSA was driven mainly by electrostatic force. The process of binding was a spontaneous process in which Gibbs free energy change was negative. Further more,the effect of luteolin on the conformation of BSA was investigated using synchronous fluorescence spectroscopy.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".