Study on Characterizations of Immobilized S-adenosylmethionine Synthetase on Chitosan Microspheres
Bibliographic record
Abstract
S-adenosylmethionine(SAM) synthetase was expressed in E. coli,and then the purified SAM synthetase was immobilized on chitosan microspheres with glutaraldehyde crosslinking.Properties of the immobilized enzyme were identified. The results showed the optimal conditions for the immobilization of the enzyme were as follows:the mass fraction of chitosan was 2.5%;the volume fraction of glutaraldehyde was 0.8%;the amount of enzyme was 6 mg/mL gel;the immobilization time was 12 hours. Under these conditions,the activity yield of the immobilized enzyme was 76%. The immobilized enzyme showed a good stability compared with the free enzyme.After incubation at 55 ℃ for 5 h the immobilized enzyme maintained 53% of the original activity while the free enzyme lost all activity at 50 ℃ for 5 h. The immobilized enzyme also showed a good stability in alkaline solution. It still kept more than 80% of the original activity when incubated in the buffer of pH 7.5~9.0 at 4 ℃ for 10 h. The immobilized enzyme was employed to synthesize the SAM and it remained 64% activity after five times repeated operations. The substrate conversion rate was more than 95% in the presence of 30 mmol/L adenosine triphosphate(ATP)。
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".