Prokaryotic expression of recombinant human bFGF and production of high titer anti-serum against bFGF
Bibliographic record
Abstract
Objective To prepare anti-human basic fibroblast growth factor (hbFGF) serum using recombinant hbFGF as an immunogen. Methods The prokaryotic expression vector for hbFGF was constructed using 5' terminal 12 codons modified by PCR, and was expressed in Escherichia coli (E. coli ). The high titer anti-hbFGF serum was prepared by immunizing New Zealand rabbits with purified hbFGF, and then analyzed by immunoblotting assay. Results The modified gene of hbFGF was highly expressed in E. coli. The recombinant hbFGF was purified (95%) from the soluble part and this recombinant protein was used to immunize rabbits. The titer of the antiserum after the second booster immunization was 1∶512 000 as determined by indirect ELISA. Immunoblotting analysis showed that this antiserum had specific reaction with both standard hbFGF and the recombinant hbFGF expressed in E. coli , and even had weak cross-reactivity with some bacterial proteins. This weak cross-reactivity was abolished after treating the antiserum with bacterial lysates. Conclusion High titer antiserum against hbFGF is produced using purified recombinant hbFGF as the immunogen and the weak cross-reactivity of the anti-serum can eliminated by bacterial lysate absorption.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".