Development of a SSR Molecular Marker for Puccinia graminis f.sp. tritici
Bibliographic record
Abstract
【Objective】The objective of this study is to build a simple,quick and accurate molecular technique to detect Puccinia graminis f.sp.tritici and forecast this destructive disease.【Method】A microsatellite-enriched genomic library of Pgt was constructed by using the method of FIASCO.Based on the sequence of Pgt microsatellite,pairs of specific SSR primers were developed and screened.【Result】The primer Pgtfssr1(f/r) generated a polymorphic pattern displaying a 395 bp DNA fragment specific for Pgt,whereas no DNA fragment was obtained in other 24 non-target wheat fungal pathogens.The existence of specific DNA fragment was detected in the infected wheat tissues at 30 h post-inoculation,and the sensitivity of this molecular marker was Pgt DNA template of 1 ng·μL-1.【Conclusion】A specific SSR marker for the detection of wheat stem rust has been successfully developed,which could be used for the early diagnosis and forecast of wheat stem rust.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".