Studies on the Interaction of DNA with Tryptamine Modified Hypocrellin and its Rare Earth Metal Coordination Polymers
Bibliographic record
Abstract
Interaction of tryptamine modified hypocrellin(DTrpHA)and its coordination polymers(Y3+-DTrpHA,La3+-DTrpHA)with DNA,including calf thymus DNA(CT DNA) and G-quadruplex 22AG,is studied by UV-Vis absorption spectroscopy,fluorescence spectroscopy and circular dichroism(CD)spectroscopy.The results indicate that the groove binding are the major mode for the association of CT DNA with DTrpHA and its coordinaiton polymer.Large hypochromicity( 45%)and red-shift in the absorption peak of tryptamine group(≥4 nm)in DTrpHA and its coordination polymers are observed,suggesting an external end-stacking mode involved.However,less hypochromicity and no obvious red-shift in the absorption peak of hypocrellin moiety in DTrpHA and its coordination polymers,suggesting that the binding mode of hypocrellin moiety could involve non-specific interactions to loop bases or sugar-phosphate backbone of G-quadruplex.G-quadruplex 22AG maintains intramolecular antiparallel structure before and after association with DTrpHA or its coordination polymers.Y3+-DTrpHA has stonger interaction towards G-quadruplex structure than DTrpHA and La3+-DTrpHA.Y3+-DTrpHA can slightly increase the melting temperature(Tm)of CT DNA by only 1.9℃,but increase the Tm of G-quadraplex by 13.1℃.Fluorescent intercalator displacement(FID)assay results demonstrate that Y3+-DTrpHA possesses high affinity towards G-quadruplex with low G4 DC50 value(the required concentration of ligand or complex that the fluorescence reduced 50%in TO/G-quadruplex system)and displays good selectivity for G-quadruplex over duplex DNA.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".