Isolation and Sequence Analysis on Puroindoline Genes of Grain Hardness in Psathyrostachys Huashanica
Bibliographic record
Abstract
The Puroindoline a(HM 448475)and Puroindoline b(HM 448476) genes of Psathyrostachys huashanica were isolated by AS-PCR method.Nucleotide sequence analysis showed that the similarity in these two sequences was only 69.25%,and the identity of these two sequences with the Puroindoline gene of relative species,such aTriticum,Aegilops,Secale,Avena,and Hordeum so on,were more than 93%.Deduced amino acid sequence analysis demonstrated that the sequence HM 448475(Pin a) and HM 448476(Pin b) all had the typical structural features of Puroindoline gene in cereal crop,containing the signal peptide of 19 amino acid residues,the tryptophan motif of WRWWKWWK(Pin a) or WPTKWWK(Pin b),and the location fixed 10 cysteine residues.Meanwhile,compared with the amino acid sequence of Puroindoline gene in relative species,the two sequences HM 448475(Pin a) and HM 448476(Pin b) had fifteen and eight major sites difference,respectively.Phylogenetic tree analysis indicated that the sequence(HM 448475) might be a new type of Pin a gene family,and sequence(HM 448476) might has a relatively near homologous with Hordoindoline b gene(GU 214830) of Hordeum chilense.This result suggested that P.huashanica contain the different Puroindoline gene alleles to control the grain hardness.The studies had important significance for enriching and improving wheat resources of grain hardness,and for protecting and exploiting the advantageous genes of the endangered species P.huashanica.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".