Screening, mutation breeding and medium optimization of glucan-producing lactic acid bacteria
Bibliographic record
Abstract
One lactic acid bacteria strain 2-17 with the glucan-producing capacity of(5.24±0.14) g/L was isolated from the commercial pickles and the pickles made in the laboratory. This strain was identifi ed as Leuconostoc mesenteroides subsp. mesenteroides by a series of physiological and biochemical tests and analysing the 16 SrDNA sequence. The glucan-producing capacity of the strain was improved by exposing the strain to nitrosoguanidine and UV irradiation successively, and the optimal mutagenic condition was determined as the following: the strain was first cultured in the MRS medium for 9h and then was exposed to 0.5 mg/m L nitrosoguanidine for 80 minutes followed by exposure to UV radiation(30 W) from distance of 45.0 cm for 30 s; then the culture was treated by the same nitrosoguanidine-UV complex mutation again. After that, one mutant UN2-18 with the glucan-producing capacity of(7.54±0.08) g/L was obtained and the glucan production of this mutant was 43.89% higher than that of its original strain. In addition, the strain obtained by continuous subculturing the mutant UN2-18 for 10 generations leveled out its glucan production by around 7.5 g/L. The optimal culture conditions of the cheap medium were determined for 2% soybean meal hydrolysised with degree of 10%, 10% sucrose and 2% K2HPO4. The glucan production of UN2-18 was(34.4±0.07) g/L, improving by 3.56 fold.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".