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Record W2383679740

GmCHS8 and GmIFS2 Gene Co-Determine Accumulation of Isoflavonoid in Soybean

2011· article· en· W2383679740 on OpenAlexaff
Yi Jin

Bibliographic record

VenueACTA AGRONOMICA SINICA · 2011
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicSoybean genetics and cultivation
Canadian institutionsAgriculture and Agri-Food Canada
Fundersnot available
KeywordsIsoflavonoidBiologyGermplasmAgrobacteriumGeneGene expressionPopulationTransformation (genetics)MicroarrayHorticultureBiotechnologyGeneticsBiochemistryFlavonoidAntioxidant
DOInot available

Abstract

fetched live from OpenAlex

Soybean isoflavonoids (isoFLVs) are natural secondary componds produced in Phenylpropanol metabolism Pathway. In past ten years, the isoFLVs were proved with anti-cancer activity to human health and therefore intensively included in nowadays food industry. However, the low content in natural soybean germplasm is a major limit to be weed in desired population. Herein we report that expression patterns of 18 362 genes were monitored by using Microarray between 30 to 70 days after pollination (DAP) during the soybean seed development. The expression profile of CHS8 and IFS2 revealed by both Microarray and RT-PCR indicated these two genes may play critical roles in determination of the accumulation of isoFLV in soybean seeds. According to Agrobacterium rhizogenes transformation system, over-expression of individuals of CHS8 and IFS2 resulted in 65.9% and 34.4% isoFLV increase respectively, but not significant in Duncan’s multiple test at the 0.05 probability level. However, co-expression of CHS8+ IFS2 significantly increased (P0.0001) the isoFLV content up to 82.3% when comparing to non-transgenic control. Conclusively, both IFS2 and CHS8 gene co-determine the accumulation of isoFLV in soybean.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.807
Threshold uncertainty score0.232

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.099
GPT teacher head0.273
Teacher spread0.174 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2011
Admission routes1
Has abstractyes

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Same venueACTA AGRONOMICA SINICASame topicSoybean genetics and cultivationFrench-language works237,207