Study on Interaction between Apigenin and Herring Sperm DNA by Acridine Orange as a Fluorescence Probe
Bibliographic record
Abstract
The interaction of apigenin with herring sperm DNA was studied with acridine orange as a fluorescence probe.The fluorescence spectra indicated that a kind of compound of apigenin and herring sperm DNA was formed at pH=7.40.The binary compound ratio was napigenin∶nDNA=2∶1;the binding constants of apigenin with herring sperm DNA compound were 1.068×104 L·mol-1(300 K) and 1.137×104 L·mol-1(310 K),respectively.The thermodynamic parameters of the interaction were calculated as follows:△rHm=1.899×103 J·mol-1,△rSm=83.475 J·mol-1·K-1,△rGm=-2.306×104 J·mol-1 at the 300 K.The interaction of apigenin with DNA was also studied through method of viscosity.The results confirmed that the intercalation model was the major mode of the interaction between apigenin and herring sperm DNA,and the binding of apigenin with herring sperm DNA was an entropy-driven reaction.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".