Optimization of RAPD and ISSR-PCR Reaction Systems for Radish (Raphanus sativus L.) Genomic DNA
Bibliographic record
Abstract
The effect of different concentrations of template DNA,primer,dNTPs and Mg2+on the PCR reaction was analyzed and the optimized RAPD and ISSR-PCR systems in radish were established.The RAPD-PCR reaction was performed in a 18 L solution containing 0.4-0.6 μmol/L of RAPD primer,0.15-0.2 mmol/L of dNTPs,2.0 mmol/L of Mg2+,genomic DNA 10-40 ng and 0.8 U TaqDNA polymerase.The ISSR-PCR reaction was performed in a 16 μl solution containing 0.5 μmol/L of primer,0.16 mmol/L of dNTPs,2.5 mmol/L of Mg2+,10-40 ng template DNA and 0.64 U TaqDNA polymerase.Gradient PCR experiment showed,The annealing temperature of 35-42 ℃ (37 ℃) and 52.4-58.3 ℃(55 ℃) are suitable for RAPD and ISSR-PCR in radish,respectively.17 radish cultivars were fingerprinted with the optimized systems.The systems would play an important role in molecular breeding,cultivar fingerprinting and seed purity testing in radish.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.004 | 0.003 |
| Meta-epidemiology (narrow) | 0.002 | 0.002 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.002 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.002 | 0.001 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.002 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".