294 B CELL MODULATION BY TOXIC SHOCK SYNDROME TOXIN-1 INDUCED CD25+ VERSUS CD25- CD4+ REGULATORY T CELLS GENERATED IN BALB/C MICE
Bibliographic record
Abstract
<h3>Purpose</h3> T regulatory (Treg) cells, comprising only 5-10% of the peripheral CD4+ T cell population, are known to suppress T cell function in the host immune response. One subtype of Tregs is the Treg-1 (Tr1) cells, which can be generated in Balb/c mice following repeated subcutaneous injection of Toxic Shock Syndrome Toxin-1 (TSST-1), a superantigen secreted by Staphylococcus aureus. The purpose of this study is to assess the modulating effects of TSST-1 induced Tregs on the proliferation and apoptosis of murine B cells in response to TSST-1. <h3>Methods</h3> 6-8 week-old female BALB/c mice (n = 6) were administered 4 μg TSST-1 or PBS subcutaneously for 3 doses at 4-day intervals. Mice were sacrificed 2 h after the last injection, and splenocytes were obtained. CD4+CD25+ and CD4+CD25- T cells were purified by magnetic separation. Both groups of CD4+ T cells were co-cultured in a 1:1 ratio with splenic B cells from naïve mice, and restimulated with 1 nM TSST-1 for 3 days in vitro. Co-cultures were treated with anti-CD19-allophycocyanin (APC) to identify the B cell population. Carboxyfluorescein diacetate succinimidyl ester (CFSE) staining was used to track B cell proliferation, and 7-amino actinomycin D (7-AAD) was employed to assess apoptosis. <h3>Results</h3> Co-culturing with PBS primed CD4+CD25- T cells in the presence of 1nM TSST-1 caused B cells to undergo at least 3 cycles of proliferation. In contrast, co-culturing with either TSST-1 primed CD4+CD25+ or CD4+CD25- T cells inhibited progression of B cell proliferation (p≤0.05; student t test). 7-AAD staining revealed that TSST-1 primed CD4+CD25+ Tregs in the presence of TSST-1 prevented B cell proliferation by inducing B cell apoptosis. On the other hand, TSST-1 primed CD4+CD25- Tregs did not cause B cell apoptosis, but prevented B cell proliferation likely by down-regulating CD19 expression. <h3>Conclusion</h3> These data provides evidence that in addition to modulating T cell activity, TSST-1 induced Tregs also regulate B cell proliferation and apoptosis in response to TSST-1 re-stimulation in vitro. Furthermore, CD4+CD25+ and CD4+CD25- Tregs appear to have different mechanisms of action. These results raises the possibility of using TSST-1 primed CD4+CD25+ or CD4+CD25- Tregs for controlling B cell-mediated autoimmune diseases, such as rheumatoid arthritis or systemic lupus erythematosis.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".