PHENOTYPIC AND MOLECULAR CHARACTERIZATION OF PATHOGENIC VIBRIO PARAHAEMOLYTICUS ISOLATED FROM PENAEUS VANNAMEI
Bibliographic record
Abstract
A mass mortality of cultured Litopenaeus vannamei broke out during summer in a local farm in northern Jiangsu Province.Syndromes include swimming near water surface and the pond walls,with black gill and opaque muscle.Tissue samples were taken in the liver,and strong pathogenicity to L.vannamei,P.japonicus and P.chinensis were determined by immersion.The phenotypic characteristics of isolates(4 strains) were examined in morphology,physiology and biochemistry;the 16S rRNA and gyrB were amplified by PCR and compared with sequences in databases,from which molecular phylogenetic trees were constructed.The sequenced 16S rRNA gene of strain JGB080708-1(GenBank accession No.GQ205448) is 1456bp in length,the sequenced gyrB gene of strain JGB080708-1(GenBank accession No.GQ205453) is 1195bp in length.Results of phylogenetic analysis on 16S rRNA and gyrB genes revealed a high homogeneity between the isolates and V.parahaemolyticus from GenBank database.Comparison in method between 16S rRNA and gyrB genes confirms that gyrB gene analysis is more useful.The isolates were identified as V.parahaemolyticus [(Fujino et al 1951) Sakazaki Nakamura and Takizawa 1963] of Vibrio(Pacini 1854) in phenotypic and molecular characteristics.Test on the activity of extracellulase and hemolysin shows that the isolates could produce diastase,gelatinase,and lecithinase,but no proteinase and lipase,and KP haemolysis was negative.Examination of K-antigen and agglutination titers show that the isolates have K-antigen,and the titer of OK-agglutinogen with OK-antiserum and O-agglutinogen with O-antiserum was the same(7log2);the titer of OK-antiserum with O-agglutinogen and O-antiserum with OK-agglutinogen was 2log2 and 3log2,respectively.The drug resistance of isolates to 49 antimicrobial agents was detected,the results show that the iso-lates are resistant to penicillin G,ampicillin,oxacillin,lincomycin,bacitracin and amoxicillin.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".