Establishment and Optimization of ISSR-PCR Reaction System of Morinda officinalis How
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Bibliographic record
Abstract
Taking genomic DNA extracted from Morinda officinalis How.leaves as the experimental material,the factors which affected the ISSR-PCR amplification such as suitable concentration for dNTPs and Mg2+,the dosage for Taq DNA polymerase,the primer,template DNA and annealing temperature were selected and optimized.The results showed that the suitable PCR conditions for ISSR-PCR of Morinda officinalis How.were as follows:In the 20 μl PCR reaction volume,0.2 mmol/L dNTPs,2.0 mmol/L Mg2+,1.0 U TaqDNA polymerase,0.5 mol/L primer,50 ng template DNA.The optimal PCR amplification process was:5 minutes at 94 ℃ for predenaturation,then followed by 45 cycles,each with 30 seconds at 94 ℃ for denaturation,45 seconds at 53.4 ℃ for annealing,1 minute at 72 ℃ for extension,finally extension at 72 ℃ for 10 minutes and holding the samples at 4 ℃.The system was applied in the amplification of six varieties of Morinda officinalis How.,indicated the suitability and stability of the system.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it